Description
Principle of the assay: human REG-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for REG-4 has been precoated onto 96-well plates. Standards(E.coli, D23-P158) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for REG-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human REG-4 amount of sample captured in plate.
Background: Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors